Immagine acquisita tramite microscopia confocale del doppio transgenico flk1:mcherry-cmyb:GFP che mostra la nicchia staminale ematopoietica nella regione caudale dell’embrione di zebrafish a 48 ore di sviluppo. In rosso (flk1:mcherry) è visibile il sistema vascolare e quindi le cellule endoteliali, mentre in verde (cmyb:GFP) sono marcate le cellule staminali ematopoietiche.
Four virtual sections of Hatinh langur (Trachypithecus hatinhensis) baculum reveal proximal-to-distal internal morphology. Micro-CT and nano-CT unveil Haversian canals, Volkmann canals, osteons, and lacunae. Local size analysis quantifies canal diameters throughout the bone, supported by histogram data. From macro to micro: a primate’s tale, one voxel at a time.
Confocal microscopy acquisition of raphe nuclei in a Tph2::GFP mouse embryo at E12.5. Triple immunofluorescence labeling was performed with antibodies against GFP, Tph2, and 5-HT. The image was digitally enhanced to boost color saturation and highlight fluorescent signals.
Composite image of Halla parthenopeia showing stereomicroscope view, longitudinal and transverse sections. Tetrachrome staining (alcian blue, periodic acid, picric acid, Weigert’s hematoxylin) clearly distinguishes epithelial, muscular, connective tissues, and nuclei, aiding morphological analysis. Notably, a mucous gland associated with feeding, unique to this marine polychaete, is highlighted (mg). Scale bar=1mm
Confocal acquisition of an adult mouse brain in coronal section. Parvalbumin-expressing neurons are stained in cyan, perineuronal net is highlighted in red, and cell nuclei are in blue.
Sezione coronale dell’occhio di una larva di zebrafish a 5 giorni dopo la fecondazione, ottenuta dalla linea transgenica Tg(gfap:GFP; reln:gal4; UAS:RFP). Le cellule gliali radiali Gfap+, in verde, si distribuiscono lungo la retina, mentre in rosso, osserviamo l’espressione di reln che contraddistingue specifiche popolazioni neuronali. Il cristallino, al centro, esprime GFP come marcatore di avvenuta transgenesi.
Neuroimaging (microscopia olotomografica)
Lysis of a mouse brain
Time-lapse Single Plane Illumination Microscopy (SPIM) imaging of a Tg(-3.1neurog1:GFP)sb2 zebrafish embryo from 48 to 72 hours post-fertilization (hpf). The embryo was xenografted with PKH26-labeled glioblastoma (GBM) cells (red) to visualize tumor behavior in vivo within the developing nervous system (GFP-labeled).